1995 Corn Gluten Meal Rate Weed Control Study - Year 6

Barbara R. Bingaman and Nick E. Christians

Corn gluten meal (CGM) is being screened for efficacy as a natural product herbicide in turf. This long-term study was begun in 1995 at the Iowa State University Horticulture Research Station north of Ames, IA. The experimental is in established 'Ram 1' Kentucky bluegrass. The soil is a Nicollet (fine-loamy, mixed, mesic Aquic Hapludoll) with an organic matter content of 4.2%, a pH of 6.85, 6 ppm P, and 120 ppm K. Prior to treatment in 1995, the percentage broadleaf weed cover within the study perimeter exceeded 50%.

Individual experimental plots are 10 x 10 ft with three replications. The experimental design is a randomized complete block design. Each year corn gluten meal is applied to the same plots at a yearly rate of 40 lb CGM/1000 ft2 (equivalent to 4 lb N/1000 ft2) using four different regimes of single and split applications for a total of five treatments (Table 1). Four applications of 10 lb/1000 ft2, split applications of 20 lb/1000 ft2, an initial application of 30 lb plus a sequential of 10 lb/1000 ft2, and a single application of 40 lb/1000 ft2 are included with an untreated control.

Initial applications for 2000 were made on April 18 before crabgrass germination. It was warm (65º) and sunny with a SW wind and the temperatures remained warm with only 0.13" rainfall for the remainder of April. The second application of treatment 2 was made on June 19 under sunny skies with a high temperature of 83º and a SW wind. The third application of treatment 2 and the second of treatments 3 and 4 were made on July 27. It was 86º, sunny and dry. There was no rain until August 4 and only 2.43" of rainfall for the entire month of August. The final application of treatment 2 was made on September 5. Temperatures remained above normal for the entire month of September and rainfall amounts were below normal.

The experimental plot was checked for phytotoxicity after each treatment. Turf quality data were taken weekly from spring greenup on April 26 through September 12. Visual quality was measured using a 9 to 1 scale with 9 = best and 6 = lowest acceptable, and 1 = worst quality (Table 1).

Crabgrass was first observed on June 27. Population data were recorded on July 14, July 25, August 2, August 8, August 16, August 24, August 29 and September 12 (Tables 2 and 3).

Broadleaf data were taken from April 26 through September 12. Dandelion and clover were the predominate broadleaf weed species within the experimental plot. Dandelion populations were measured counting the number of plants per plot Tables 4 and 5). Clover infestations were estimated by determining the percentage area in each individual plot covered by clover (Tables 6 and 7).

Data were analyzed with the Statistical Analysis System (SAS, Version 6.12) and the Analysis of Variance (ANOVA) procedure. Means comparisons were made with Fisher’s Least Significant Difference test (LSD). Crabgrass, clover, and dandelion population data were converted to percentage reductions as compared with the untreated controls (Tables 3, 5, and 7).

The 2000 growing season was extremely dry. As a result there was less response to the nitrogen in the corn gluten meal than in previous years. Supplemental irrigation was used as required to keep the bluegrass from entering dormancy. In spite of the growing conditions, turf quality was significantly better in bluegrass treated with CGM than in the untreated control for most of the season (Table 1). By September 5, the turf was dormant but rainfall in late October resulted in significant greening so additional data were taken on October 27. On this date, turfgrass that received sequential applications (treatments 2, 3, and 4) had better quality than the untreated control and treated turf that received only an initial CGM treatment. Mean visual quality for the entire season was better for bluegrass treated with CGM than the untreated grass.

Crabgrass populations were statistically reduced by CGM at all application rates as compared with the untreated control from August 2 through September 12 (Table 2). The best crabgrass control was in turf treated with split applications of 20 lb CGM but this level of control was not statistically different from the other CGM treatments. Mean reductions in crabgrass counts were > 85% in all CGM treated turf as compared with the untreated control (Table 3).

In 2000, crabgrass counts in CGM treated turf were statistically lower than in the untreated control (Table 8). Crabgrass control was better at all CGM treatment levels than in previous years. Four sequential applications of 10 lb CGM provided 85% reduction in crabgrass counts in 2000 but only 28, 0, 48, 42, and 67% in 1995, 1996, 1997, 1998, and 1999, respectively. Split applications of 20 lb CGM provided 100% crabgrass reductions in 2000 as compared to 45, 33, 50, 86, and 95% in 1995, 1996, 1997, 1998, and 1999, respectively. A single 40 lb CGM application decreased crabgrass counts by 92% in 2000.

Dandelion numbers were significantly lower in all CGM treated turf as compared with the untreated controls for the entire 2000 season (Table 4). The highest level of dandelion control (88.4% reduction) was in turf treated with split applications of 20 lb CGM but this control was not different from the other CGM treatments. Mean crabgrass reductions were similar for all CGM treatments and ranged from 77.8 to 88.4% (Table 5). Dandelion control was equal to or slightly better than control in previous years (Table 10). Dandelion reductions were 48, 50, 66, and 69% in 1996, 1997, 1998, and 1999, respectively as compared with 78% in 2000.

Clover cover fluctuated throughout the season because of the dry conditions. Clover populations were significantly lower in all CGM treated turf than in untreated on all data collection dates except May 2 and June 2 (Table 6). Mean reductions for all CGM treatments were > 94% and all were statistically different from the untreated controls (Table 7). Clover control in 2000 was approximately equal to control in 1999. At all CGM treatment levels, control was better than control in 1996, 1997 and 1998 (Table 11).

Table 1. Visual quality1 of Kentucky bluegrass treated with corn gluten meal in the 1995 Corn Gluten Meal Rate Weed Control Study (April 26 - October 27).

 

Material

Rate

lb product/1000 ft2

April

26

May

2

May

10

May

17

May

23

June

2

June

8

June

15

June

22

June

30

1.

Untreated control

NA

6.0

5.7

6.0

6.0

6.0

6.0

6.0

6.0

6.0

6.0

2.

Corn gluten meal

10 fb 10 fb 10 fb 10

8.7

8.7

7.7

7.7

7.3

7.3

7.0

7.0

7.0

7.0

3.

Corn gluten meal

20 fb 20

7.0

8.3

7.7

8.3

8.3

7.7

7.7

7.7

7.7

8.0

4.

Corn gluten meal

30 fb 10

6.7

7.7

7.3

8.3

9.0

8.3

8.7

8.3

8.7

8.7

5.

Corn gluten meal

40

6.3

6.7

8.0

8.3

9.0

8.7

8.7

8.7

9.0

8.3

 

LSD0.05

 

0.8

1.1

NS

1.0

0.7

1.1

0.8

0.9

0.7

0.8

 

 

Material

Rate

lb product/1000 ft2

July

7

July

14

July

25

Aug

2

Aug

8

Aug

24

Aug

29

Sept

5

Oct

27

Mean

1.

Untreated control

NA

6.0

6.0

6.0

6.0

6.0

6.0

6.0

6.0

6.0

6.0

2.

Corn gluten meal

10 fb 10 fb 10 fb 10

8.0

8.7

7.7

8.3

8.3

8.0

8.0

7.0

8.3

7.8

3.

Corn gluten meal

20 fb 20

7.3

7.0

7.3

7.0

7.3

8.3

8.0

7.0

8.3

7.7

4.

Corn gluten meal

30 fb 10

8.0

8.0

7.7

8.3

7.7

8.3

8.7

7.0

8.0

8.1

5.

Corn gluten meal

40

8.7

8.3

8.3

8.7

8.0

7.3

7.7

7.0

7.3

8.1

 

LSD0.05

 

1.2

0.8

NS

1.2

1.62

1.3

1.5

NS

1.6

0.5

1Visual quality was assessed using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst turf quality.

2For these data P > F=0.07

Initial applications were made on April 18. Sequential applications of treatment 2 were made on June 29, July 27, and September 5. Sequential applications of treatments 3 & 4 were made on July 27.

NS = means are not significantly different at the 0.05 level.

Table 2. Crabgrass counts1 in Kentucky bluegrass treated with corn gluten meal in the 1995 Corn Gluten Meal Rate Weed Control Study.

 

Material

Rate

lb product/1000 ft2

July

14

July

25

Aug

2

Aug

8

Aug

16

Aug

24

Aug

29

Sept

12

Mean

1.

Untreated control

NA

4.3

13.0

15.7

47.0

59.0

39.3

55.0

34.0

33.4

2.

Corn gluten meal

10 fb 10 fb 10 fb 10

5.7

1.7

1.7

10.0

6.0

5.0

5.0

4.3

4.9

3.

Corn gluten meal

20 fb 20

0.0

0.0

0.0

0.3

0.3

0.3

0.3

0.0

0.2

4.

Corn gluten meal

30 fb 10

0.0

0.0

0.0

4.3

2.7

3.3

5.3

3.3

2.4

5.

Corn gluten meal

40

1.0

0.3

2.0

3.0

3.3

2.3

4.3

5.3

2.7

 

LSD0.05

 

NS

NS

12.62

36.22

38.9

24.8

36.0

16.7

21.0

1These values represent the number of crabgrass plants per plot.

2For these data P > F = 0.08.

NS = means are not significantly different at the 0.05 level.

 

Table 3. Crabgrass count reductions1 in Kentucky bluegrass treated with corn gluten meal in the 1995 Corn Gluten Meal Rate Weed Control Study.

 

Material

Rate

lb product/1000 ft2

July

14

July

25

Aug

2

Aug

8

Aug

16

Aug

24

Aug

29

Sept

12

Mean

1.

Untreated control

NA

0.0

0.0

0.

0.0

0.0

0.0

0.0

0.0

0.0

2.

Corn gluten meal

10 fb 10 fb 10 fb 10

0.0

87.2

89.4

78.7

89.8

87.3

90.9

87.3

85.3

3.

Corn gluten meal

20 fb 20

100.0

100.0

100.0

99.3

99.4

99.2

99.4

100.0

99.5

4.

Corn gluten meal

30 fb 10

100.0

100.0

100.0

90.8

95.5

91.5

90.3

90.2

92.9

5.

Corn gluten meal

40

76.7

97.4

87.3

93.6

94.4

94.1

92.1

84.3

91.9

 

LSD0.05

 

NS

NS

80.52

77.02

65.9

63.1

65.4

49.1

63.0

1These values represent the percentage reduction in crabgrass plants per plot as compared with the untreated control.

2For these data P > F = 0.08.

NS = means are not significantly different at the 0.05 level.

Table 4. Dandelion counts1 in Kentucky bluegrass treated with corn gluten meal in the 1995 Corn Gluten Meal Rate Weed Control Study.

 

Material

Rate

lb product/1000 ft2

April

26

May

2

May

17

June

2

June

15

June

22

June

30

July

7

1.

Untreated control

NA

65.7

71.3

61.3

58.3

59.3

78.3

68.7

68.3

2.

Corn gluten meal

10 fb 10 fb 10 fb 10

17.0

19.7

9.3

16.3

15.7

23.0

17.3

17.7

3.

Corn gluten meal

20 fb 20

10.7

11.3

8.3

9.0

7.7

9.0

7.7

6.3

4.

Corn gluten meal

30 fb 10

18.0

21.3

11.7

16.3

13.7

18.0

13.0

13.7

5.

Corn gluten meal

40

18.0

17.7

11.3

15.0

13.7

24.3

14.7

14.3

 

LSD0.05

 

32.4

31.1

29.8

25.2

21.6

34.0

25.3

24.1

 

 

Material

Rate

lb product/1000 ft2

July

14

July

25

Aug

2

Aug

8

Aug

24

Aug

29

Sept

12

Mean

1.

Untreated control

NA

61.7

68.0

68.7

67.3

54.3

48.7

56.3

63.8

2.

Corn gluten meal

10 fb 10 fb 10 fb 10

9.7

14.7

14.7

10.3

8.0

9.0

9.7

14.1

3.

Corn gluten meal

20 fb 20

6.3

6.0

7.3

5.3

5.0

3.7

7.0

7.4

4.

Corn gluten meal

30 fb 10

6.7

11.0

12.0

7.0

7.3

8.7

12.0

12.7

5.

Corn gluten meal

40

9.0

11.3

12.3

9.3

9.0

10.7

12.7

13.6

 

LSD0.05

 

17.5

28.9

27.5

28.3

16.5

26.1

20.9

24.4

1These data represent the number of dandelion plants per plot.

Table 5. Dandelion count reductions1 in Kentucky bluegrass treated with corn gluten meal in the 1995 Corn Gluten Meal Rate Weed Control Study.

 

Material

Rate

lb product/1000 ft2

April

26

May

2

May

17

June

2

June

15

June

22

June

30

July

7

1.

Untreated control

NA

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

2.

Corn gluten meal

10 fb 10 fb 10 fb 10

74.1

72.4

84.8

72.0

73.6

70.6

74.8

74.1

3.

Corn gluten meal

20 fb 20

83.8

84.1

86.4

84.6

87.1

88.5

88.8

90.7

4.

Corn gluten meal

30 fb 10

72.6

70.1

81.0

72.0

77.0

77.0

81.1

80.0

5.

Corn gluten meal

40

72.6

78.2

81.5

74.3

77.0

68.9

78.7

79.0

 

LSD0.05

 

49.3

43.6

48.7

43.3

36.4

43.4

36.8

35.3

 

 

Material

Rate

lb product/1000 ft2

July

14

July

25

Aug

2

Aug

8

Aug

24

Aug

29

Sept

12

Mean

1.

Untreated control

NA

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

2.

Corn gluten meal

10 fb 10 fb 10 fb 10

84.3

78.4

78.7

84.6

85.3

81.5

82.8

77.8

3.

Corn gluten meal

20 fb 20

89.7

91.2

89.3

92.1

90.8

92.5

87.6

88.4

4.

Corn gluten meal

30 fb 10

89.2

83.8

82.5

89.6

86.5

82.2

78.7

80.1

5.

Corn gluten meal

40

85.4

83.3

82.0

86.1

83.5

78.1

77.5

78.8

 

LSD0.05

 

28.4

42.4

40.0

42.1

30.3

53.5

37.1

38.3

1These values represent the percentage reductions in dandelion counts per plot as compared with the untreated control.

Table 6. Percentage clover cover1 in Kentucky bluegrass treated with corn gluten meal in the 1995 Corn Gluten Meal Rate Weed Control Study.

 

Material

Rate

lb product/1000 ft2

April

26

May

2

May

17

June

2

June

15

June

22

June

30

July

7

1.

Untreated control

NA

0.7

3.7

30.0

13.7

13.3

13.3

30.0

38.3

2.

Corn gluten meal

10 fb 10 fb 10 fb 10

0.0

0.3

0.0

0.7

0.0

0.7

0.3

0.3

3.

Corn gluten meal

20 fb 20

0.0

1.7

0.0

0.3

0.7

0.0

0.7

2.0

4.

Corn gluten meal

30 fb 10

0.0

0.3

0.0

0.3

0.3

0.3

0.7

2.3

5.

Corn gluten meal

40

0.0

0.3

2.0

0.7

0.3

0.7

1.0

0.3

 

LSD0.05

 

0.5

NS

16.6

NS

8.6

8.7

14.9

16.8

 

 

Material

Rate

lb product/1000 ft2

July

14

July

25

Aug

2

Aug

8

Aug

24

Aug

29

Sept

12

Mean

1.

Untreated control

NA

15.0

21.7

31.7

12.7

38.3

33.3

35.0

22.0

2.

Corn gluten meal

10 fb 10 fb 10 fb 10

0.3

0.0

1.7

1.7

0.7

0.3

1.7

0.6

3.

Corn gluten meal

20 fb 20

0.3

0.3

0.7

1.0

0.0

0.7

2.0

0.7

4.

Corn gluten meal

30 fb 10

2.0

2.0

0.7

0.6

1.7

2.0

3.3

1.1

5.

Corn gluten meal

40

0.7

0.3

2.0

1.7

3.3

1.7

1.7

1.1

 

LSD0.05

 

7.4

8.6

7.0

7.8

10.4

12.9

8.1

6.9

1These figures represent the percentage of each plot covered by clover.

NS = means are not significantly different at the 0.05 level.

Table 7. Percentage clover cover reductions1 in Kentucky bluegrass treated with corn gluten meal in the 1995 Corn Gluten Meal Rate Weed Control Study.

 

Material

Rate

lb product/1000 ft2

April

26

May

2

May

17

June

2

June

15

June

22

June

30

July

7

1.

Untreated control

NA

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

2.

Corn gluten meal

10 fb 10 fb 10 fb 10

100.0

91.0

100.0

95.1

100.0

95.0

98.9

99.1

3.

Corn gluten meal

20 fb 20

100.0

55.0

100.0

97.6

95.0

100.0

97.8

94.8

4.

Corn gluten meal

30 fb 10

100.0

91.0

100.0

97.6

97.5

97.5

97.8

93.9

5.

Corn gluten meal

40

100.0

91.0

93.3

95.1

97.5

95.0

96.7

99.1

 

LSD0.05

 

69.5

NS

55.2

NS

64.9

65.6

49.7

43.8

 

 

Material

Rate

lb product/1000 ft2

July

14

July

25

Aug

2

Aug

8

Aug

24

Aug

29

Sept

12

Mean

1.

Untreated control

NA

0.0

0.0

0.0

0.0

0.0

0.0

0.0

0.0

2.

Corn gluten meal

10 fb 10 fb 10 fb 10

97.8

100.0

94.7

84.9

98.3

99.0

95.2

97.4

3.

Corn gluten meal

20 fb 20

97.8

98.5

97.9

92.1

100.0

98.0

94.3

96.9

4.

Corn gluten meal

30 fb 10

86.7

90.8

97.9

97.4

95.6

94.0

90.5

95.1

5.

Corn gluten meal

40

95.6

98.5

93.7

86.9

91.3

95.0

95.2

94.9

 

LSD0.05

 

49.5

39.5

22.0

61.7

27.0

38.7

23.3

31.3

1These figures represent the percentage reductions in clover cover per plot as compared to the untreated control.

NS = means are not significantly different at the 0.05 level.

Table 8. Crabgrass counts1 in Kentucky bluegrass treated in the 1995 Corn Gluten Meal Rate Weed Control Study for 1996 through 2000.

 

Material

Rate

lb product/1000 ft2

1996

1997

1998

1999

2000

1.

Untreated control

NA

4

36

19

102

33

2.

Corn gluten meal

10 fb 10 fb 10 fb 10

7

19

11

33

5

3.

Corn gluten meal

20 fb 20

3

18

3

5

0

4.

Corn gluten meal

30 fb 10

1

14

4

17

2

5.

Corn gluten meal

40

5

37

9

10

3

 

LSD0.05

 

NS

NS

NS

NS

21

1These values represent the number of crabgrass plants per plot.

NS = means are not significantly different at the 0.05 level.

 

Table 9. Percentage crabgrass count reductions1 in Kentucky bluegrass treated in the 1995 Corn Gluten Meal Rate Weed Control Study for 1995 through 2000.

 

Material

Rate

lb product/1000 ft2

1995

1996

1997

1998

1999

2000

1.

Untreated control

NA

0

0

0

0

0

0

2.

Corn gluten meal

10 fb 10 fb 10 fb 10

28

0

48

42

67

85

3.

Corn gluten meal

20 fb 20

45

33

50

86

95

100

4.

Corn gluten meal

30 fb 10

44

67

61

78

86

93

5.

Corn gluten meal

40

54

0

0

53

90

92

 

LSD0.05

 

NS

NS

NS

NS

NS

63

1These values represent the percentage reductions in crabgrass plants per plot as compared with the untreated controls.

NS = means are not significantly different at the 0.05 level.

 

Table 10. Percentage dandelion count reductions1 in Kentucky bluegrass treated in the 1995 Corn Gluten Meal Rate Weed Control Study for 1996 through 2000.

 

Material

Rate

lb product/1000 ft2

1996

1997

1998

1999

2000

1.

Untreated control

NA

0

0

0

0

0

2.

Corn gluten meal

10 fb 10 fb 10 fb 10

48

50

66

69

78

3.

Corn gluten meal

20 fb 20

50

60

59

85

88

4.

Corn gluten meal

30 fb 10

28

28

69

76

80

5.

Corn gluten meal

40

50

58

62

79

79

 

LSD0.05

 

NS

NS

21

38

38

1These figures represent the percentage reductions in dandelion counts per plot as compared to the untreated control.

NS = means are not significantly different at the 0.05 level.

 

Table 11. Percentage clover cover reductions1 in Kentucky bluegrass treated in the 1995 Corn Gluten Meal Rate Weed Control Study for 1996 through 2000.

 

Material

Rate

lb product/1000 ft2

1996

1997

1998

1999

2000

1.

Untreated control

NA

0

0

0

0

0

2.

Corn gluten meal

10 fb 10 fb 10 fb 10

45

65

74

96

97

3.

Corn gluten meal

20 fb 20

69

82

72

99

97

4.

Corn gluten meal

30 fb 10

90

92

64

99

95

5.

Corn gluten meal

40

92

83

93

100

95

 

LSD0.05

 

NS

58

49

71

31

1These figures represent the percentage reductions in clover cover per plot as compared to the untreated control.

NS = means are not significantly different at the 0.05 level.