Hort 423/523
Plant Tissue Culture
General Information
Fall 2007
Library reserve
Instructor: Loren Stephens, 127 Hort, ph. 4-1917, e-mail
Course Objectives: First, to become familiar with the principal methods employed in plant tissue culture, and their current and potential uses in agriculture and the biological sciences. Secondly, to understand the underlying principles that allow these methods to be successfully used. Thirdly, to gain some practical experience in plant tissue culture.
Course Structure. This is a 2-credit class that will meet each Tuesday at 1:10 pm for lecture/discussion. Each student also will have arranged lab times during the week for independent lab work.
Expected Outcomes. At the end of the semester, you should be able to: disinfest and place into culture suitable explants capable of being cultured and multiplied, make culture medium from reagent grade chemicals and stock solutions, routinely transfer cultures without contamination, and analyze the usefulness of information available from the scientific literature that deals with plant tissue culture.
